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Serum protein electrophoresis (SPEP)

Also called: SPEP, Protein electrophoresis, Free light chains

SPEP separates the proteins in your blood. It's used to look for an abnormal antibody (M-protein) made by multiple myeloma and related conditions.

SampleA small tube of blood from a vein in your arm
Fasting?Usually not needed
ResultsSeveral days
Good to knowOne result rarely tells the whole story. Your provider looks at your results together with your symptoms, exam, and other tests.

What the test is

A small M-protein without symptoms (MGUS) is common in older adults and is usually just monitored.

Why it's done

  • Unexplained anemia, kidney problems, high calcium, or bone pain
  • High total protein

Getting ready and what happens

  • No special preparation is needed.

How the test is done

  • A health worker cleans the skin, puts a band around your arm, and draws blood from a vein with a small needle. It takes a few minutes.

What the results mean

M-spike found

More tests, like free light chains and sometimes a bone marrow test, decide between MGUS, myeloma, and other conditions.

Questions to ask your provider

  • What do my results mean for me?
  • Do I need to repeat this test or have other tests?

Risks

A blood draw is very safe. You may have slight pain or a small bruise where the needle went in.

Related conditions

Related tests

Sources

Last updated 2026-11-13.