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Mono test

Also called: Monospot, Heterophile antibody test, EBV antibody test

A mono test checks for infectious mononucleosis, usually caused by the Epstein-Barr virus.

SampleBlood
Fasting?Usually not needed
ResultsMinutes to a few days
Good to knowOne result rarely tells the whole story. Your provider looks at your results together with your symptoms, exam, and other tests.

What the test is

The rapid monospot can be falsely negative in the first week and in young children. EBV antibody tests are more detailed.

Why it's done

  • Sore throat, fever, swollen glands, and severe tiredness, often in teens and young adults

Getting ready and what happens

  • No special preparation is needed.

How the test is done

  • A health worker cleans the skin, puts a band around your arm, and draws blood from a vein with a small needle. It takes a few minutes.
  • Some clinics use a finger prick.

What the results mean

Positive

Supports mono. Avoid contact sports for at least 3 to 4 weeks because the spleen can rupture.

Negative early on

May be repeated later.

When a result needs quick action

Call 911Sudden sharp pain in the upper left belly or shoulder, or fainting (possible spleen rupture)

Questions to ask your provider

  • What do my results mean for me?
  • Do I need to repeat this test or have other tests?
  • When can I return to sports?
  • How long will I feel tired?

Risks

A blood draw is very safe. You may have slight pain or a small bruise where the needle went in.

Related conditions

Related tests

Sources

Last updated 2026-11-13.